Luminescence (Lumi)
The photons generated by the luminescence reaction are converted
into electrical signals for detection by PMT in the reader. The signal
collected by instrument covers the entire spectrum and allows to do
glow-type luminescence. Luminescent detection provides high sens-
itivity and wide dynamic range to the assay,which does not require a
light source or specific optics for excitation, thus it is optically simpler
than fluorescence detection.
Light-induced chemiluminescence (LiCA)
LiCA technology is a homogeneous affinity detection method based
on a pair of microbeads that can be used for almost all research
applications. LiCA no-wash assays provide simple, rapid,and highly
sensitive detection of biomolecules in cell lysates,cell supernatants,
serum,and various other types of samples,as well as binding assays
with a broad affinity range.LiCA detection technology can complete
96-well plate detection in minutes,while maintaining a very high
signal-to-background ratio,enabling fast, highly sensitive detection
techniques.
Time-resolved fluorescence(TRF)
TRF provides higher signal-to-noise ratios,excellent sensitivity&stability,
wider dynamic range,as well as flexibility,even in limited or very low
sample volumes.TRF detection uses the fluorescence retention effect of
lanthanide chelates to reduce the interference of background fluorescence
in the reaction that significantly improve the dynamic range and has
high signal stability.
● Using dual-mode photon counting acquisition and correction
technology,through the patented photon counting linear fitting
technology,the commonly used photon counter with a dynamic
range of 10^5 can be expanded to 10^7,expanding the dynamic/
linear range by 100-fold and adapting to a wider range of
concentration gradient experiments.
● The con-focal optical system can effectively suppress the
interference of ambient stray light,and minimize the cross
-interference caused by the difference in high and low
concentrations between wells.
● In the TRF detection mode,the combination of dual
excitation optical paths and high dynamic range PMT
technology can ensure that weak signals are detected,
while strong signals do not enter the saturation state,
and the maximum dynamic range and linear range can
be obtained at one timeIn the LiCA mode,laser is used
for excitation, which can minimize the influence of non
-specific fluorescence in the microplate or reaction sample
excited by other light.
● With a built-in incubator for temperature control up to 45°C,
iP96 provides a uniform incubation and prevents condensation.
It is well-suited for temperature-critical applications,including
certain enzyme assays and cell-based applications.
● iP96 comes with an analysis software,its easy-to navigate
interface will guide you through the measurement process
and help you get the results you need.A variety of application
schemes and experimental templates are preset in the software,
suitable for most common applications.Support different
measurement modes such as standard curve quantification,
end-point method, kinetic analysis, etc.
● iP96 has power-on self-test and auto-calibration functions for reliable results.
Automatic dynamic range, which selects optimal reading range based on the signal intensity
The instrument supports 8*12 opaque microplate and automation-friendly plate carrier guarantee an easy integration into robotic platform.
Small footprint meets the requirements of small space configuration, saving the laboratory space as much as possible.